甲状腺激素受体α在猪有腔卵泡发育和孤雌激活胚胎发育过程中表达模式

doi:10.11843/j.issn.0366-6964.2014.06.007

Abstract

Abstract:

Expression pattern of TR alpha in different developmental stages of porcine follicules，in vitro matured oocytes and early embryos were systemically investigated in the present study.The THs concentration of the different diameter sizes of pig follicular was determined by chemiluminescence immunoassay (CLIA) technique.And the localization of TR alpha in pig ovary were determined by immunohistochemical techniques.The expression pattern of TR alpha in pig oocytes and pre-implantation embryos were determined with immunofluorescence and QRT-PCR.The results showed that T3 concentration in pig follicles was rised at first and then declined following the increase of follicle diameter.TR alpha-immunoreactive substance was mainly distributed over granular cells，theca cells and oocytes．The quantity of the TR alpha-immunoreactive and TR alpha-immunoreactive cells were increased following the development of follicles．The results of QRT-PCR showed that TR alpha was expressed through out the process of in vitro matured oocytes，and the relative expression level in 1-cell，2-cell and 4-cell stages were significant higher than that of 8-cell morula and blastula stage.Immunohistochemical analysis results showed that TRa clearly expressed following the increase of follicles，and reached peak at 24-36 h.TR alpha protein expressed in different stages of early embryos，and its expression level was upregulated at 8-cell，morula and blastocyst stage.THs existed in the pig follicle fluid，TR alpha expressed in different stages of COCs and early embryos，THs may be affected pig folliculogenesis and early embryonic development by banding with the TR alpha.

CLC Number:

S823.3

ZHONG Jun-jie,YANG Gui-fang,LIU Shuai，LIU Qing-you,XIE Bing-kun,CUI Kui-qing,SHI De-shun. Expression Pattern Analysis of Thyroid Hormone Receptor Alpha in Pig Antral Follicles and Parthenogenetic Early Embryos[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(6): 901-909.

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References

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Expression Pattern Analysis of Thyroid Hormone Receptor Alpha in Pig Antral Follicles and Parthenogenetic Early Embryos

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